The Alberta Implementation Guidebook translates implementation science into a practical, step-by-step resource for organizations and teams seeking to implement evidence-based programs, practices, or system changes. Organized around four implementation phases, Explore, Plan & Prepare, Implement, and Sustain, the guidebook includes checklists, guiding questions, core activities, checkpoints, and curated resources to support successful implementation. Although developed for Alberta’s healthcare system, the framework and tools can be broadly applied across prevention, public health, education, community-based, and human services settings. The guidebook also emphasizes engagement, co-design, equity, and sustainability throughout the implementation process.